Purification and characterization of recombinant Rtt109, a fungus-specific histone acetyltransferase, from Candida albicans

Posted by dbos UCSD / Salk

  Kavli Affiliate: V. S. Ramachandran | Authors: Simran Sharma, Vijayan Ramachandran, Sneha Sudha Komath, Rohini Muthuswami and Samudrala Gourinath | Summary: Epigenetic regulation of chromatin dynamics via histone acetylation is one of several mechanisms by which eukaryotes regulate gene expression, DNA replication and repair, and maintain genome stability. This function is performed by histone […]


Continue.. Purification and characterization of recombinant Rtt109, a fungus-specific histone acetyltransferase, from Candida albicans

High Axial Resolution Is Necessary for Quantitative Two-Photon Calcium Imaging of Neuronal Populations

Posted by dbos Hopkins

  Kavli Affiliate: Adam S. Charles | Authors: Ha Yun Anna Yoon, Umaima Afifa, Génesis Ferrer-Imbert, Adam S Charles and Na Ji | Summary: Two-photon calcium imaging is a standard tool for measuring neuronal population activity in vivo, yet how axial resolution, sensor expression strategy, and analysis pipeline jointly affect data accuracy remains poorly characterized. Here, […]


Continue.. High Axial Resolution Is Necessary for Quantitative Two-Photon Calcium Imaging of Neuronal Populations

Microglia extract neuronal proteolytic organelles via skoupocytosis – Copy

Posted by dbos Hopkins

  Kavli Affiliate: Reza Shadmehr | Authors: Jafar Doostmohammadi, Nazanin Mohammadrezaei, Hisham Elseweifi, Alana Chandler, Elijah Taeckens and Reza Shadmehr | Summary: Neurons in the brain are often many synapses away from motoneurons, yet if a movement results in error, each distant neuron needs a teacher that considers its specific contribution to production of that […]


Continue.. Microglia extract neuronal proteolytic organelles via skoupocytosis – Copy

Social isolation recruits amygdala-medial prefrontal cortex projections to escalate alcohol drinking in male mice

Posted by dbos UCSD / Salk

  Kavli Affiliate: Kay Tye | Authors: Reesha Patel, Kelly Kim, Makenzie Patarino, Rachelle Pamintuan, Felix Taschbach, Hao Li, Bitna Joo, Anna Pallé, Xianru Yu, Christopher R Lee, Aniek van Hoek, Jesse White, Rogelio Castro, Christian Cazares, Raymundo Miranda, Caroline Jia, Jeremy Delahanty, Kanha Batra, Laurel Keyes, Avraham Libster, Romy W. Wichmann, Talmo Pereira, Marcus […]


Continue.. Social isolation recruits amygdala-medial prefrontal cortex projections to escalate alcohol drinking in male mice

TLR4 signaling drives tissue inflammation, Claudin-5 internalization, and vascular barrier breakdown in a mouse model of neonatal meningitis

Posted by dbos Hopkins

Kavli Affiliate: Jeremy Nathans | Authors: Philip V Seegren, Amir Rattner, Philip M Smallwood and Jeremy Nathans | Summary: Neonatal bacterial meningitis is a leading cause of infant morbidity and mortality, yet the molecular and cellular basis of the leptomeningeal response to infection remains poorly defined. Here, we study a mouse model of neonatal E. […]


Continue.. TLR4 signaling drives tissue inflammation, Claudin-5 internalization, and vascular barrier breakdown in a mouse model of neonatal meningitis

PLCβs are recruited to the plasma membrane in macrophages by both Gβγ and Gαq

Posted by dbos Rockefeller

Kavli Affiliate: Roderick MacKinnon | Authors: Maria Falzone, Priyam Banerjee and Roderick MacKinnon | Summary: PLCβ enzymes cleave PIP2 from the plasma membrane, producing IP3 and DAG, which regulate intracellular Ca2+ levels and protein kinase C activity, respectively. They are regulated by GPCR signaling through the G proteins Gβγ and Gαq and have been shown […]


Continue.. PLCβs are recruited to the plasma membrane in macrophages by both Gβγ and Gαq

Orchestrating Spatial Transcriptomics Analysis with Bioconductor

Posted by dbos Hopkins

Endothelial type I interferon signaling modulates the vascular response to ischemic brain injury Kavli Affiliate: Keri Martinowich | Authors: Helena L Crowell, Yixing Dong, Ilaria Billato, Peiying Cai, Martin Emons, Samuel Gunz, Boyi Guo, Mengbo Li, Alexandru Mahmoud, Artür Manukyan, Hervé Pagès, Pratibha Panwar, Shreya Rao, Callum J Sargeant, Lori Shepherd Kern, Marcel Ramos, Jieran […]


Continue.. Orchestrating Spatial Transcriptomics Analysis with Bioconductor

An Integrated Multi-omics Single Cell Atlas of the Human RPE and Choroid

Posted by dbos Hopkins

  Kavli Affiliate: Seth Blackshaw | Authors: Jianming Shao, Xuan Bao, Ismail Yaman, Ye Zheng, Jun Wang, Jin Li, Tingting Yang, Jinjing Jian, Jean Li, Seth Blackshaw, Kapil Bharti, Margo Clarke, Dwight E. Stambolian, Ching-Hwa Sung, Jie J. Zheng, Davide Ortolan, Sarah X. Zhang, Ruchi Sharma, Richard H. Scheuermann, Ajith V. Pankajam, Ahmed H. Ghobashi, […]


Continue.. An Integrated Multi-omics Single Cell Atlas of the Human RPE and Choroid

Orthogonal CRISPR screens and human liver chimeric mice identify hepatitis B virus host factors

Posted by dbos Rockefeller

  Kavli Affiliate: Charles M. Rice | Authors: Catherine A Freije, Georgios Dangas, Antonis Athanasiadis, Evgenia Moschogianni, Madeleine K Sanders, Allan Henrique Depieri Cataneo, Chloe K Boehm, Maria Bousali, Dar-Yin Li, Sooyoung Lee, Guillaume Cornelis, Gary Lo, Leah B Soriaga, Amalio Telenti, Julia di Iulio, Ana L P Mosimann, Juliano Bordignon, Kirin Karver, Lukas Fu, […]


Continue.. Orthogonal CRISPR screens and human liver chimeric mice identify hepatitis B virus host factors

Spatial deconstruction of the plasma membrane

Posted by dbos Rockefeller

Endothelial type I interferon signaling modulates the vascular response to ischemic brain injury Kavli Affiliate: Roderick MacKinnon | Authors: Venkata Shiva Mandala, Chen Zhao, Qiuying Chen, Steven Gross, Jacob B Geri and Roderick MacKinnon | Summary: The plasma membrane of cells is known to be heterogeneous with regards to the spatial distribution of proteins and […]


Continue.. Spatial deconstruction of the plasma membrane