Kavli Affiliate: Jeanne Paz | Authors: Isaac Y.M. Chang and Jeanne T. Paz | Summary: Coordinated movement relies on interactions between the basal ganglia and the thalamus, yet the circuits linking these structures are not fully understood. Here, we dissect a non-canonical basal ganglia output pathway, from the external globus pallidus (GPe) to the nucleus […]
Kavli Affiliate: Alex Pollen | Authors: Joana Rocha, Runyang Nicolas Lou, Carolina De Lima Adam, Prajna Hebbar, Scott Ferguson, Davide Bolognini, Alison Killilea, Kendra Hoekzema, Andrea Guarracino, Yun Deng, Nicole Soranzo, Benedict Paten, Erik Garrison, Alex Pollen, Evan Eichler, Rori Rohlfs, Matthew Mitchell and Peter H Sudmant | Summary: Complete, haplotype-resolved genome assemblies have provided […]
Kavli Affiliate: Arturo Alvarez-Buylla | Authors: Mercedes F Paredes, Oier Pastor-Alonso, Matthew Heffel, Mohammad S Baig, Jasmine Harris, Susana Gonzalez Granero, Siqian Li, Sol Beccari, Julia Chu, Hannah Lambing, I-Ling Lu, Matthew Varughese, Andrew L Cheng, Jessica Le, Mohini Bhade, JaeYeon Kim, Arantxa Cebrian-Silla, Isabel Torres Cuevas, Kurtis I Auguste, Eric Huang, Arturo Alvarez-Buylla, Jesse […]
Kavli Affiliate: Lily Jan | Authors: Yayu Wang and Lily Yeh Jan | Summary: This protocol describes manual whole-endolysosome patch-clamp recordings from pharmacologically or genetically enlarged endolysosomes in cultured mammalian cells. Steps include vesicle enlargement, fabrication and fire-polishing of high-resistance pipettes, mechanical dissection and isolation of enlarged vesicles, giga-seal formation, and configuration to whole-endolysosome modes. […]
Kavli Affiliate: Mazen Kheirbek | Authors: Mark M Gergues, Gregory I Telian, Kelsey Logas, Mona Li, Arsine Kolanjian, Jeremy S Biane, Shazreh Hassan, Jasmine Tai, Victoria S Turner and Mazen A Kheirbek | Summary: Adaptive behavior requires neural circuits that encode salient stimuli to guide approach and avoidance. The hippocampal ventral subiculum (vSub) encodes information […]
Kavli Affiliate: Robert Edwards | Authors: Severin Coleon, Rasangi Kankanam Pathirage, Wenge Ding, Elizabeth Van Itallie, Katayoun Mansouri, Lorie Marchitto, Derek W. Cain, Xiaozhi Lu, Varsha Kumari, Laura Sutherland, Robert Parks, Andrew Foulger, Thingochuong Le, Lena Smith, Joena Bal, Esther Lee, Alecia Brown, Fangping Cai, Savanna A. Toure, Rebecca Williams, Madison Berry, Ashwin Skelly, Edward […]
Kavli Affiliate: Yifan Cheng | Authors: Junrui Li, Wooyoung Choi, Yifei chen, Shawn Zheng, Angus McDonald, John W. Sedat, David A. Agard and Yifan Cheng | Summary: With technological advancements in recent years, single particle cryogenic electron microscopy (cryo-EM) has become a major methodology for structural biology. Structure determination by single particle cryo-EM is premised […]
Kavli Affiliate: Loren Frank | Authors: Shijie Gu, Chenyan Liu, Anna K. Gillespie, Rhino Nevers, Eric L. Denovellis, Michael E. Coulter, Kenneth Kay and Loren M. Frank | Summary: Changing one’s mind –– revising a past decision independently of external cues –– can lead to better outcomes, but its neural basis remains poorly understood. We […]
Kavli Affiliate: Robert Edwards | Authors: Susanna R Grigson, Natia Geliashvili, Torsten Schubert, George Bouras, Vijini Mallawaarachchi, Marta Bogacz, Ute Hellmich, Robert A Edwards and Bas E. Dutilh | Summary: Bacteriophages (phages) play essential roles in microbial systems, yet most phage proteins remain poorly characterised. Protein tertiary and quaternary structure information contributes valuable information about […]
Kavli Affiliate: Devanand Manoli | Authors: David M Young, Ruchira Sharma, Narjes Rohani, Chimmi Dema, Lindsay Liang, Bernie Devlin, Devanand S. Manoli and Stephan J SandersH. Janak | Summary: The past decade has seen tremendous progress in the identification of genes associated with complex neuropsychiatric disorders, including autism spectrum disorder (ASD) and schizophrenia. Expression patterns […]