Kavli Affiliate: Charles M. Rice | Authors: Trinity Zang, Viren A Baharani, Miranda Aldis, Marie Canis, Rachel Patejak, Edmund Osei Kuffour, Hans Heinrich Hoffman, Harm van Bakel, Emilia M Sordillo, Viviana Simon, Margaret R MacDonald, Charles M Rice, Michel C Nussenzweig, Theodora Haziionannou and Paul D Bieniasz | Summary: A central problem in achieving vaccine-based […]
Kavli Affiliate: Sarah Woolley | Authors: Erin M Wall, Nicholas Vidas-Guscic, Jasmien Orije, Elisabeth Jonckers, Marleen Verhoye, Annemie Van der Linden and Sarah Cushing Woolley | Summary: Social relationships profoundly shape the perception of communication signals. In animals that form long-term social bonds, sensory cues such as vocalizations, scent, or physical appearance, and rewarding mating […]
Kavli Affiliate: Adam S. Charles, Michael Miller, and Richard Huganir | Authors: Shashwat Kumar, Gabrielle I Coste, Dasun Premathilaka, Richard L. Huganir, Austin R Graves, Adam S Charles and Michael I Miller | Summary: Synapses are the fundamental unit of neural connectivity, exhibiting dynamic functional and structural changes that enable the brain to learn, adapt, […]
Kavli Affiliate: Kenta Asahina | Authors: Linghao Hu, Pingchuan Ma, Vaibhav Menon, Athira Sethu Madhavan, Kenta Asahina, Lena M. Müller and Adam J. Bowman | Summary: Fluorescence lifetime microscopy is limited by photon throughput, constraining the speed and dynamic range of biological measurements. We demonstrate a compact optical module and fast phasor acquisition methods to […]
Kavli Affiliate: Lily Jan | Authors: Yayu Wang and Lily Yeh Jan | Summary: This protocol describes manual whole-endolysosome patch-clamp recordings from pharmacologically or genetically enlarged endolysosomes in cultured mammalian cells. Steps include vesicle enlargement, fabrication and fire-polishing of high-resistance pipettes, mechanical dissection and isolation of enlarged vesicles, giga-seal formation, and configuration to whole-endolysosome modes. […]
Kavli Affiliate: Ann-Shyn Chiang | Authors: Heng Chang, Yueh-Feng Wu, Ching-Che Charng, Kai-Chun Jhan, Shun-Chi Wu, Ann-Shyn Chiang, Hsiao-Chun Amy Lin, Chung-Chuan Lo, Bi-Chang Chen, Sung-Jan Lin and Li-An Chu | Summary: Volumetric intravital imaging of adult Drosophila brains remains challenging due to optical scattering and speed limitations of conventional microscopy. Here, we present V-shape […]
Kavli Affiliate: Michael Miller | Authors: Qi Wang, Debarshi Banerjee, Erin Connolly, Claire Vanpouille-Box, Carrie Shawber, Rami Vanguri, Michael Kissner, Michael L. Miller, Darrell J. Yamashiro and Eileen P. Connolly | Summary: Background High-dose radiotherapy (RT) in cancer is immunogenic but also induces an immunosuppressive tumor microenvironment (TME) that limits the efficacy of immune checkpoint […]
Kavli Affiliate: Dhakshin Ramanathan and Jyoti Mishra | Authors: Morteza Salimi, Milad Nazari, Anna Linnik, Shruti Joshi, Jonathan Mishler, Ryan Golden, Maxim Bazhenov, Jennifer Rodger, Sahar Jomehpour, Miranda Francoeur Koloski, Jyoti Mishra and Dhakshin Ramanathan | Summary: Intermittent Theta Burst Stimulation (iTBS) is a patterned stimulation protocol FDA-cleared to treat depression, yet its outcomes are […]
Kavli Affiliate: Mazen Kheirbek | Authors: Mark M Gergues, Gregory I Telian, Kelsey Logas, Mona Li, Arsine Kolanjian, Jeremy S Biane, Shazreh Hassan, Jasmine Tai, Victoria S Turner and Mazen A Kheirbek | Summary: Adaptive behavior requires neural circuits that encode salient stimuli to guide approach and avoidance. The hippocampal ventral subiculum (vSub) encodes information […]
Kavli Affiliate: Scott Small | Authors: Yukun A Hao, Lorna Louise Jayne, Sungmoo Lee, Mia Nicole Dittrich, Manze Zhang, Simon Haziza, Imane Bendifallah, Ruth R. Sims, Boris Bouazza-Arostegui, Alexander D White, John Kochalka, Yu Wang, Maedeh Seyedolmohadesin, Adrian Negrean, Zhaoyang Li, Collin Chiu, Kaspar Podgorski, Jun B. Ding, Karl Deisseroth, Rafael Yuste, VALENTINA EMILIANI, Mark […]