Kavli Affiliate: Dwight Bergles | Authors: Yuqing Yan, Jiaxi Lu, Zhe Li, Zuo-Han Zhao, Timothy F. Shay, Shunzhi Wang, Yaping Lei, Yimei Wang, Wei Chen, Patrick Parker, Hongru Yang, Aileen Qi, Yongzhi Sun, Dwight Bergles, David Baker and Dingchang Lin | Summary: Mapping cellular activity with high spatiotemporal precision in complex tissues is essential for […]
Kavli Affiliate: Joshua Vogelstein | Authors: Tingshan Liu, Jayanta Dey, Beiya Xu, Samuel S Alldritt, Karl-Heinz Nenning, Kyoungseob Byeon, Ting Xu and Joshua T. Vogelstein | Summary: Sex classification using neuroimaging data has the potential to revolutionize personalized diagnostics by revealing subtle structural brain differences that underlie sex-specific disease risks. Despite the promise of machine […]
Kavli Affiliate: Justus M Kebschull | Authors: Hyopil Kim, Cheng Xu, Craig Washington, Maggie Lowman and Justus M Kebschull | Summary: Brain-wide neural circuits are formed by the diverse axonal branching patterns of many individual neurons. Here we introduce POlNTseq (projections of interest by sequencing), a high-throughput and user-friendly barcoded connectomics method that uses cell […]
Kavli Affiliate: Richard Huganir | Authors: Hana Goldschmidt Merrion, Casey N. Barber, Santosh S. Renuse, Jevon Cutler, Simion Kreimer, Alexei M. Bygrave, David J. Meyers, William Dylan Hale, Akhilesh Pandey and Richard L. Huganir | Summary: Synaptic plasticity in the central nervous system enables the encoding, storing, and integrating new information. AMPA-type glutamate receptors (AMPARs) […]
Kavli Affiliate: Seth Blackshaw | Authors: Haley Appel, Rogger Carmen-Orozco, Clayton Santiago, Thanh Hoang and Seth Blackshaw | Summary: The RNA-binding protein Ptbp1 has been proposed as a master regulator of neuronal fate, repressing neurogenesis through its effects on alternative splicing and miRNA maturation. While prior studies using RNA interference suggested that Ptbp1 loss promotes […]
Kavli Affiliate: Justus M Kebschull | Authors: Hyopil Kim, Cheng Xu, Craig Washington, Maggie Lowmann and Justus M Kebschull | Summary: Brain-wide neural circuits are formed by the diverse axonal branching patterns of many individual neurons. Here we introduce POINTseq (projections of interest by sequencing), a high-throughput and user-friendly barcoded connectomics method that uses cell […]
Kavli Affiliate: Adam S. Charles | Authors: Sai Koukuntla, Tate DeWeese, Alexandra Cheng, Robyn Mildren, Aamna Lawrence, Austin R Graves, Jennifer Colonell, Timothy D Harris and Adam S Charles | Summary: The growing channel count of silicon probes has substantially increased the number of neurons recorded in electrophysiology (ephys) experiments, rendering traditional manual spike sorting […]
Kavli Affiliate: Justus M Kebschull | Authors: Hyopil Kim, Huihui Qi, Craig Washington, Xiaoping Liang and Justus M Kebschull | Summary: The barcoded connectomics tool MAPseq enables highly multiplexed projection mapping of individual neurons by translating neuroanatomy into a DNA sequencing problem. Here we present MAPseq2, a user-friendly protocol with 3-4 fold increased barcode detection […]
Kavli Affiliate: Richard Huganir | Authors: William Dylan Hale, Richard L. Huganir and Edward C Twomey | Summary: AMPA-subtype glutamate receptors (AMPARs), composed of subunits GluA1-4, mediate fast, excitatory synaptic transmission in the brain. After glutamate binding, AMPAR ion channels exhibit multiple subconductance states that tune neuronal responses to glutamate. GluA4 is the rarest subunit […]
Kavli Affiliate: Laurent Younes and Michael Miller | Authors: Kaitlin Stouffer, Xiaoyin Chen, Hongkui Zeng, Benjamin Charlier, Laurent Younes, Alain Trouve and Michael I Miller | Summary: Advancements in imaging and molecular techniques enable the collection of subcellular-scale data. Diversity in measured features, resolution, and physical scope of capture across technologies and experimental protocols pose […]