Kavli Affiliate: Barbara Baird | First 5 Authors: Sarah Veatch, Benjamin Machta, Sarah Shelby, Ethan Chiang, David Holowka | Summary: We present an analytical method to quantify clustering in super-resolution localization images of static surfaces in two dimensions. The method also describes how over-counting of labeled molecules contributes to apparent self-clustering and how the effective […]

Kavli Affiliate: Gerald W. Feigenson | First 5 Authors: Frederick A. Heberle, Gerald W. Feigenson, , , | Summary: We have developed a new model to describe FRET efficiency (E FRET) between freely-diffusing membrane probes in phase-separated bilayers (Finite Phase-separation FRET, or FP-FRET), that in principle applies to any system where phase domain dimensions are […]

Kavli Affiliate: Gerald W. Feigenson | First 5 Authors: Frederick A. Heberle, Gerald W. Feigenson, , , | Summary: Multicomponent lipid mixtures exhibit complex phase behavior, including coexistence of nanoscopic fluid phases in ternary mixtures mimicking the composition of the outer leaflet of mammalian plasma membrane. The physical mechanisms responsible for the small size of […]

Kavli Affiliate: Gerald W. Feigenson | First 5 Authors: Frederick A. Heberle, David G. Ackerman, Gerald W. Feigenson, , | Summary: The presence and the properties of lipid bilayer nanometer-scale domains might be important for understanding the membranes of living cells. We used molecular dynamics (MD) simulations to investigate perturbations of a small patch of […]