Microdissection and culturing of adult lateral entorhinal cortex layer II neurons from APP/PS1 Alzheimer model mice

Posted by klaurent NTNU

Kavli Affiliate: Menno Witter, Asgeir Kobro-Flatmoen | Authors: Katrine Sjaastad Hanssen, Menno P. Witter, Axel Sandvig, Ioanna Sandvig and Asgeir Kobro-Flatmoen | Summary: Abstract Background Primary neuronal cultures enable cell-biological studies of Alzheimer’s disease (AD), albeit typically non-neuron-specific. The first cortical neurons affected in AD reside in layer II of the lateralmost part of the […]


Continue.. Microdissection and culturing of adult lateral entorhinal cortex layer II neurons from APP/PS1 Alzheimer model mice

Structural basis for CaVα2δ:gabapentin binding

Posted by klaurent UCSF

Kavli Affiliate: Daniel Minor | Authors: Daniel L Minor, Jr., Zhou Chen and Abhisek Mondal | Summary: Abstract Gabapentinoid drugs for pain and anxiety act on the CaVα2δ-1 and CaVα2δ-2 subunits of high-voltage activated calcium channels (CaV1s and CaV2s). Here, we present the cryo-EM structure of the gabapentin-bound brain and cardiac CaV1.2/CaVβ3/CaVα2δ-1 channel. The data […]


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Microbial symbionts are shared between ants and their associated beetles

Posted by klaurent Rockefeller

Kavli Affiliate: Daniel Kronauer | Authors: Catalina Valdivia, Justin A. Newton, Sean O’Donnell, Christoph von Beeren, Daniel J. C Kronauer, Jacob A Russell and Piotr Lukasik | Summary: Abstract The transmission of microbial symbionts across individuals and generations can be critical for animal development and survival. Likewise, the transmission of microbes across closely interacting species […]


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Unclearing Microscopy

Posted by klaurent Yale

Kavli Affiliate: Joerg Bewersdorf | Authors: Ons M’Saad, Michael Shribak and Joerg Bewersdorf | Summary: Abstract The spatial resolution and contrast sensitivity of the human eye is limited, restricting our ability to directly see subcellular structures. We report a new principle for unaided eye cellular visualization in a method we call Unclearing Microscopy. By expanding […]


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Automated head-fixation training system with high levels of animal participation in psychoacoustic tasks

Posted by klaurent Hopkins

Kavli Affiliate: Patrick Kanold | Authors: Ji Liu, Kate Maximov and Patrick O Kanold | Summary: Abstract Many animal training paradigms rely on head-fixation. Head-fixation training is typically laborious and can benefit from automation to relieve the workload as well as to reduce the variability in the training outcome. Several groups have reported successful implementations […]


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A selectivity filter in the EMC limits protein mislocalization to the ER

Posted by klaurent NTNU

Kavli Affiliate: Vy Thuy Nguyen | Authors: Tino Pleiner, Masami Hazu, Giovani Pinton Tomaleri, Vy N. Nguyen, Kurt Januszyk and Rebecca M. Voorhees | Summary: SUMMARY Tail anchored proteins (TAs) play essential roles at both the ER and mitochondria, and their accurate localization is critical to proteostasis. Biophysical similarities lead to mistargeting of mitochondrial TAs […]


Continue.. A selectivity filter in the EMC limits protein mislocalization to the ER

Long-range inhibitory axons from medial entorhinal cortex target lateral entorhinal neurons projecting to the hippocampus

Posted by klaurent NTNU

Kavli Affiliate: Bente Jacobsen, Menno Witter, Eirik Nilssen | Authors: Eirik S. Nilssen, Bente Jacobsen, Thanh P. Doan, Paulo J.B. Girao and Menno P. Witter | Summary: ABSTRACT Functionally distinct information encoded by the two main divisions of the entorhinal cortex (EC), the lateral EC (LEC) and the medial EC (MEC), is thought to be […]


Continue.. Long-range inhibitory axons from medial entorhinal cortex target lateral entorhinal neurons projecting to the hippocampus

Violation of the ultrastructural size principle in the dorsolateral prefrontal cortex underlies working memory impairment in the aged common marmoset (Callithrix jacchus)

Posted by klaurent UCSD / Salk

Kavli Affiliate: John Reynolds | Authors: Courtney Glavis-Bloom, Casey R Vanderlip, Sammy Weiser Novak, Masaaki Kuwajima, Lyndsey Kirk, Uri Manor, Kristen M Harris and John H Reynolds | Summary: ABSTRACT Working memory relies critically on the dorsolateral prefrontal cortex (dlPFC). Morphology and function of the dlPFC, and corresponding working memory performance, are affected early in […]


Continue.. Violation of the ultrastructural size principle in the dorsolateral prefrontal cortex underlies working memory impairment in the aged common marmoset (Callithrix jacchus)

Generative network modeling reveals quantitative definitions of bilateral symmetry exhibited by a whole insect brain connectome

Posted by klaurent Hopkins

Kavli Affiliate: Carey Priebe, Joshua Vogelstein | Authors: Benjamin D. Pedigo, Mike Powell, Eric W. Bridgeford, Michael Winding, Carey E. Priebe and Joshua T. Vogelstein | Summary: Abstract Comparing connectomes can help explain how neural connectivity is related to genetics, disease, development, learning, and behavior. However, making statistical inferences about the significance and nature of […]


Continue.. Generative network modeling reveals quantitative definitions of bilateral symmetry exhibited by a whole insect brain connectome

BDNF and TRiC-inspired Reagents Rescue Cortical Synaptic Deficits in a Mouse Model of Huntington’s Disease

Posted by klaurent UCSD / Salk

Kavli Affiliate: William Mobley | Authors: Yingli Gu, Alexander Pope, Charlene Smith-Geater, Christopher Carmona, Aaron Johnstone, Linda Shi, Xuqiao Chen, Sarai Santos, Claire C Bacon-Brenes, Thomas Shoff, Korbin M Kleczko, Judith Frydman, Leslie M Thompson, William C Mobley and Chengbiao Wu | Summary: Abstract Huntington’s disease (HD) results from a CAG repeat expansion in the […]


Continue.. BDNF and TRiC-inspired Reagents Rescue Cortical Synaptic Deficits in a Mouse Model of Huntington’s Disease