Kavli Affiliate: Achillefs Kapanidis | Summary:Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards ensuring both the reproducibility and accuracy of measuring FRET efficiencies are currently lacking. Here we report the results of a worldwide, […]

Kavli Affiliate: Vladyslav Vyazovskiy | Summary:Increasing evidence suggests that cortical dynamics during wake exhibits long-range temporal correlations suitable to integrate inputs over extended periods of time to increase the signal-to-noise ratio in decision-making and working memory tasks. Accordingly, sleep has been suggested as a state characterized by a breakdown of long-range correlations; detailed measurements of […]

Kavli Affiliate: Stephan Rauschenbach | Summary:Imaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics and molecular nanotechnology. In particular, revealing the distinct conformations of an individual protein is of utmost importance. Here, we show the imaging of individual proteins and protein complexes by […]

Kavli Affiliate: Simon Newstead | Summary:Negative emotional responses to the daily life stresses have cumulative effects which, in turn, impose wide-ranging negative constraints on emotional well being and neurocognitive performance (Kalueff:2007cp, Charles:2013eq, Nadler:2010hk). Crucial cognitive functions such as memory and problem solving, as well more short term emotional responses (e.g., anticipation of- and response to- […]

Kavli Affiliate: David Ray | Summary:Sir, We write as senior scientists about a problem vital to the scientific enterprise and prosperity. Nowadays, funding is a lengthy and complex business. First, universities themselves must approve all proposals for submission. Funding agencies then subject those that survive to peer review, a process by which a few researchers, […]

Kavli Affiliate: Richard Berry | Summary:Recent experiments on the bacterial flagellar motor have shown that the structure of this nanomachine, which drives locomotion in a wide range of bacterial species, is more dynamic than previously believed. Specifically, the number of active torque-generating units (stators) was shown to vary across applied loads. This finding invalidates the […]

Kavli Affiliate: Richard Berry | Summary:The bacterial flagellar motor (BFM) is responsible for driving bacterial locomotion and chemotaxis, fundamental processes in pathogenesis and biofilm formation. In the BFM, torque is generated at the interface between transmembrane proteins (stators) and a rotor. It is well-established that the passage of ions down a transmembrane gradient through the […]

Kavli Affiliate: Richard Berry | Summary:We report new statistical time-series analysis tools providing significant improvements in the rapid, precision extraction of discrete state dynamics from large databases of experimental observations of molecular machines. By building physical knowledge and statistical innovations into analysis tools, we demonstrate new techniques for recovering discrete state transitions buried in highly […]

Kavli Affiliate: Carol Robinson | Summary:Computer-based tests with randomly generated questions allow a large number of different tests to be generated. Given a fixed number of alternatives for each question, the number of tests that need to be generated before all possible questions have appeared is surprisingly low. | Search Query: arXiv Query: search_query=au:”Robinson Carol”&id_list=&start=0&max_results=10Read […]