Kavli Affiliate: Molly Stevens | Summary:Unsupervised estimation of the dimensionality of hyperspectral microspectroscopy datasets containing pure and mixed spectral features, and extraction of their representative endmember spectra, remains a challenge in biochemical data mining. We report a new versatile algorithm building on semi-nonnegativity constrained self-modeling curve resolution and information entropy, to estimate the quantity of […]

Kavli Affiliate: Colin Espie | Summary:SleepioTM is a digital mobile phone and web platform that uses techniques from cognitive behavioural therapy (CBT) to improve sleep in people with sleep difficulty. As part of this process, Sleepio captures data about the sleep behaviour of the users that have consented to such data being processed. For neural […]

Kavli Affiliate: Stephan Rauschenbach | Summary:Electrospray ion-beam deposition (ES-IBD) is a versatile tool to study structure and reactivity of molecules from small metal clusters to large protein assemblies. It brings molecules gently into the gas phase where they can be accurately manipulated and purified, followed by controlled deposition onto various substrates. In combination with imaging […]

Kavli Affiliate: Philipp Kukura | Summary:Measuring the electrophoretic mobility of molecules is a powerful experimental approach for investigating biomolecular processes. A frequent challenge in the context of single-particle measurements is throughput, limiting the obtainable statistics. Here, we present a molecular force sensor and charge detector based on parallelised imaging and tracking of tethered double-stranded DNA […]

Kavli Affiliate: Philipp Kukura | Summary:Single particle tracking has found broad applications in the life and physical sciences, enabling the observation and characterisation of nano- and microscopic motion. Fluorescence-based approaches are ideally suited for high-background environments, such as tracking lipids or proteins in or on cells, due to superior background rejection. Scattering-based detection is preferable […]

Kavli Affiliate: Molly Stevens | Summary:There is a growing trend in molecular and synthetic biology of using mechanistic (non machine learning) models to design biomolecular networks. Once designed, these networks need to be validated by experimental results to ensure the theoretical network correctly models the true system. However, these experiments can be expensive and time […]

Kavli Affiliate: Stephan Rauschenbach | Summary:Using electrospray ion beam deposition, we collide the complex molecule Reichardt’s Dye (C41H30NO+) at low, hyperthermal translational energy (2-50 eV) with a Cu(100) surface and image the outcome at single-molecule level by scanning tunneling microscopy. We observe bond-selective reaction induced by the translational kinetic energy. The collision impulse compresses the […]

Kavli Affiliate: Achillefs Kapanidis | Summary:Single-molecule Förster resonance energy transfer (smFRET) has become a mainstream technique for probing biomolecular structural dynamics. The rapid and wide adoption of the technique by an ever-increasing number of groups has generated many improvements and variations in the technique itself, in methods for sample preparation and characterization, in analysis of […]

Kavli Affiliate: Philipp Kukura | Summary:We present a novel optical transient absorption and reflection microscope based on a diffraction-limited pump pulse in combination with a wide-field probe pulse, for the spatio-temporal investigation of ultrafast population transport in thin films. The microscope achieves a temporal resolution down to 12 fs and simultaneously provides sub-10 nm spatial […]

Kavli Affiliate: Achillefs Kapanidis | Summary:The fluorescent nitrogen-vacancy (NV) defect in diamond has remarkable photophysical properties, including high photostability, which allows stable fluorescence emission for hours; as a result, there has been much interest in using nanodiamonds (NDs) for quantum optics and biological imaging. Such applications have been limited by the complexity and heterogeneity of […]