Kavli Affiliate: Andrew Baldwin | Summary: We compare phase-only control with phase-and-amplitude control when designing shaped pulses using the GRAPE algorithm, and explain why phase-only control has significant advantages. Trotterisation can be used to simulate amplitude modulation with phase modulation, indicating that the two approaches are fundamentally equivalent. Pulses designed by either method can be […]
Kavli Affiliate: Andrew Baldwin | Summary: We compare phase-only control with phase-and-amplitude control when designing shaped pulses using the GRAPE algorithm, and explain why phase-only control has significant advantages. Trotterisation can be used to simulate amplitude modulation with phase modulation, indicating that the two approaches are fundamentally equivalent. Pulses designed by either method can be […]
Kavli Affiliate: Zameel Cader | Authors: Xuewei Qi, Alessandra Granata, Tom Van Agtmael, Sanjay Sinha, Zameel Cader, Hugh S Markus, Stuart M. Allan, Karen Horsburgh and Tao Wang | Summary: Cerebral small vessel disease (cSVD) is a major contributor to stroke and cognitive decline, ultimately leading to vascular dementia (VaD). Genetic factors play a key […]
Kavli Affiliate: Stephan Rauschenbach | Summary:Improving the detailed understanding of the underlying properties and functions of biomolecules has recently attracted growing interest, enabled by the possibility of real-space imaging of single, intact macromolecules using Scanning Tunneling Microscopy (STM) in combination with electrospray ion beam deposition and soft landing. This combination provides key insights into biomolecular […]
Kavli Affiliate: Andrew Baldwin | Summary:The universality of quantum theory has been questioned ever since it was proposed. Key to this long-unsolved question is to test whether a given physical system has non-classical features. Here we connect recently proposed witnesses of non-classicality, based on information-theoretic ideas, with the theory of temporal entanglement. We provide a […]
Kavli Affiliate: Justin Benesch | Summary:Size and shape are critical discriminators between molecular species and states. We describe a micro-chip based high-throughput imaging approach offering rapid and precise determination of molecular properties under native solution conditions. Our method detects differences in molecular weight across at least three orders of magnitude, and down to two carbon […]
Kavli Affiliate: Lindsay Baker | Summary:A protein’s structure in the gas phase underpins the interpretation of native mass spectrometry. Yet how fold and conformation respond to dehydration has never been resolved at the residue level, due to the unavailability of a method to image gas-phase proteins at near-atomic resolution. Here, we determine near-atomic-resolution cryoEM structures […]
Kavli Affiliate: Carol Robinson | Summary:Electrospray ion beam deposition (ESIBD) is the intact, chemically selective deposition of molecular ions on surfaces in vacuum. Here, we present a general method and dedicated instrumentation for ESIBD-based cryoEM sample preparation of soluble proteins. Precise control over deposition energy, sample environment, and reproducible growth of thin, homogeneous, vitreous ice […]
Kavli Affiliate: Lindsay Baker | Summary:Soft-landing electrospray ion beam deposition (ESIBD), i.e. the chemically selective, intact deposition of large and complex molecules on surfaces in vacuum, is a well-established sample preparation method for scanning probe microscopy (SPM), where it facilitates the high-resolution imaging of large and complex molecules. Inherited from mass spectrometry, the advantageous properties […]
Kavli Affiliate: Andrew Baldwin | Summary:Biomolecular condensates composed of intrinsically disordered proteins (IDPs) are vital for proper cellular function, and their dysfunction is associated with diseases including neurodegeneration and cancer. Despite their biological importance, the precise physical mechanisms underlying condensate (dys)function are unclear, in part owing to the difficulties in understanding how biomolecular sequence patterns […]