Kavli Affiliate: Changhuei Yang
| First 5 Authors: Shi Zhao, Shi Zhao, , ,
| Summary:
Fourier ptychographic microscopy (FPM) is a powerful computational imaging
modality that achieves high space-bandwidth product imaging for biomedical
samples. However, its adoption is limited by slow data acquisition due to the
need for sequential measurements. Multiplexed FPM strategies have been proposed
to accelerate imaging by activating multiple LEDs simultaneously, but they
typically require careful parameter tuning, and their lack of effective
aberration correction makes them prone to image degradation. To address these
limitations, we introduce hybrid-illumination multiplexed Fourier ptychographic
microscopy (HMFPM), which integrates analytic aberration extraction capability
with the efficiency of multiplexed illumination. Specifically, HMFPM employs a
hybrid illumination strategy and a customized reconstruction algorithm with
analytic and optimization methods. This hybrid strategy substantially reduces
the number of required measurements while ensuring robust aberration correction
and stable convergence. We demonstrate that HMFPM achieves 1.08 micrometers
resolution, representing a 4-fold enhancement over the system’s coherent
diffraction limit, across a 1.77×1.77 millimeter square field of view using 20
measurements. HMFPM remains robust under diverse aberrations, providing up to
84 micrometers digital refocusing capability, and effectively corrects both
field-dependent and scanning-induced aberrations in whole-slide pathology
imaging. These results establish HMFPM as a practical, high-throughput, and
aberration-free solution for biological and biomedical imaging.
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