Kavli Affiliate: Changhuei Yang
| First 5 Authors: Oumeng Zhang, Changhuei Yang, , ,
| Summary:
Single-shot fluorescence imaging techniques have gained increasing interest
in recent years due to their ability to rapidly capture complex biological data
without the need for extensive scanning. In this letter, we introduce polarized
Fourier light field microscopy (pFLFM), a novel fluorescence imaging technique
that captures five-dimensional information (3D intensity and 2D polarization)
in a single snapshot. This technique combines a polarization camera with an
FLFM setup, significantly improving data acquisition efficiency. We
experimentally validated the pFLFM system using a fluorescent Siemens star,
demonstrating consistent resolution and an extended depth of field across
various polarizations. Using the 5D imaging capabilities of pFLFM, we imaged
plant roots and uncovered unique heterogeneities in cellulose fibril
configurations across various root sections. These results not only highlight
the potential of pFLFM in biological and environmental sciences, but also
represent a significant advancement in the design of single-shot fluorescence
imaging systems.
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