Kavli Affiliate: Justus M Kebschull
| Authors: Hyopil Kim, Huihui Qi, Craig Washington, Xiaoping Liang and Justus M Kebschull
| Summary:
The barcoded connectomics tool MAPseq enables highly multiplexed projection mapping of individual neurons by translating neuroanatomy into a DNA sequencing problem. Here we present MAPseq2, a user-friendly protocol with 3-4 fold increased barcode detection sensitivity and ∼10 fold decreased cost relative to the current MAPseq protocol. As MAPseq workflows are used across a range of barcoded connectomics methods, including BARseq, BRICseq, and ConnectID, all improvements in MAPseq2 directly transfer to these technologies.