| Endothelial type I interferon signaling modulates the vascular response to ischemic brain injury |
Kavli Affiliate: Winrich Freiwald
| Authors: Nelson J. Johansen, Yuanyuan Fu, Matthew Schmitz, Alma Dubuc, Niklas Kempynck, Morgan Wirthlin, Aaron D. Garcia, Madeleine Hewitt, Meghan A. Turner, Stephanie C. Seeman, Brian Long, Xiao-Ping Liu, Shu Dan, Michael DeBerardine, Inkar Kapen, Anna Marie Yanny, Alida Avola, Samuel T. Barlow, Darren Bertagnolli, Ashwin Bhandiwad, Agata Budzillo, Victor E. Nieto Caballero, Lakme Caceres, Tamara Casper, Anish B. Chakka, Rushil Chakrabarty, Michael Clark, Scott Daniel, Jeroen Eggermont, Rebecca Ferrer, Leon French, Jessica Gloe, Jeff Goldy, Nathan Guilford, Junitta Guzman, Daniel Hirschstein, Windy Ho, Katelyn James, Danielle L. Jones, Matthew Jungert, Madhav Kannan, Kasia Z. Kedzierska, Thomas Kroes, Mckaila Leytze, Arena Manning, Rachel McCue, Chris Morrison, Beagan Nguy, Sven Otto, Nick Pena, Trangthanh Pham, Elliot Phillips, Abhejit Rajagopal, Christine Rimorin, Andrea D. Rivera, Dana Rocha, Raymond Sanchez, Geoffrey Schau, Jessica Schembri, Nadiya V. Shapovalova, Jamie Sherman, Julian Thijssen, Michael Tieu, Amy Torkelson, Alex Tran, Alexander Vieth, Yongqi Wang, Dan Yuan, Jennie Close, Tanya L. Daigle, Rachel Dalley, Nick Dee, Song-Lin Ding, Elysha Fiabane, Nathan Gouwens, Grace Huynh, Brian R. Lee, Boaz P. Levi, Delissa McMillen, Jeremy A. Miller, Tyler Mollenkopf, Lydia Ng, Patrick L. Ray, Cassandra Sobieski, Staci A. Sorensen, Zizhen Yao, Faraz Yazdani, Winrich Freiwald, Boudewijn Lelieveldt, Brian Kalmbach, C. Dirk Keene, Jesse Gillis, David Osumi-Sutherland, Jonathan T. Ting, Hongkui Zeng, Kimberly Smith, Fenna M. Krienen, Rebecca D. Hodge, Ed S. Lein and Trygve E. Bakken
| Summary:
TThe basal ganglia are interconnected subcortical nuclei with complex topographical organization that orchestrate goal-directed behaviors and are implicated in neurodegenerative movement disorders. We generated a cellular-resolution, spatial transcriptomic atlas of the basal ganglia in human, rhesus macaque, and common marmoset, sampling over one million cells in each species. By integrating spatial data with a cross-species, consensus snRNA-seq cell type taxonomy, this atlas reveals conserved principles of molecular organization within and across structures. The cellular architecture is complex but highly stereotyped, with gene expression gradients superimposed onto discrete compartments. Extensive spatial sampling illuminates 3D gradients of molecular organization in the striatum and reveals cell type-specific core and shell compartments in the primate internal globus pallidus, which is conserved with mouse. This unified, cross-species spatial transcriptomic atlas will be a foundational resource for characterizing the molecular and functional organization of the basal ganglia and their roles in health and disease.